During last night's show at Aqua Night Club in Jacksonville, Lil Wayne killed any speculation that he and Birdman have resolved their differences and made it very clear he's still at odds with his "daddy."

After plugging his upcoming 'Free Weezy Album' and 'Carter V,' Wayne sent a solid diss at his record label.

The shot comes a few weeks after Wayne referred to Birdman as his "daddy b*tch as*" during a Young Money cypher.

Check it out up top.